Phenotypic classification of gastric signet ring cell carcinoma and its relationship with clinicopathologic parameters and prognosis

Table 1 Antibodies for immunohistochemistry

Antibody	Clone	Diluent’sproportion	Company
Gastric phenotypic markers
MUC5AC	CLH2	1:100	Novocastra, Newcastle, UK
MUC6	CLH5	1:50	Novocastra, Newcastle, UK
HGM	45M1	1:50	Novocastra, Newcastle, UK
Intestinal phenotypic markers
MUC2	Ccp58	1:100	Novocastra, Newcastle, UK
CDX2	CDX2-88	1:50	BioGenex, San Ramon CA
Li-cadherin	Clone 141713	1:200	Neomarker, Fremont, CA, USA
VILLIN	CWWB1	1:200	Novocastra, Newcastle, UK
Other antibodies
Ki -67	K-2	1:50	Zymed, San Francisco, CA, USA
CD44v6	VFF-7	1:50	Zymed , San Francisco, CA, USA
E-cadherin	4A2C7	1:50	Zymed , San Francisco, CA, USA
β-catenin	CAT-5H10	1:30	Zymed , San Francisco, CA, USA