Detection of let-7a microRNA by real-time PCR in gastric carcinoma

doi:10.3748/wjg.v13.i20.2883

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May 28, 2007 (publication date) through May 7, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. May 28, 2007; 13(20): 2883-2888
Published online May 28, 2007. doi: 10.3748/wjg.v13.i20.2883

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Figure 2 Total RNA sensitivity and dynamic range of let-7a miRNA assay. A: Amplification plot of total RNA from gastric mucosa at six orders of magnitude. The total RNA input ranged from 50 pg to 5 μg in per stem-loop RT-PCR reaction; B: Correlation of total RNA input with the threshold of cycle (Ct) values for let-7a miRNA assays.

Citation: Zhang HH, Wang XJ, Li GX, Yang E, Yang NM. Detection of let-7a microRNA by real-time PCR in gastric carcinoma. World J Gastroenterol 2007; 13(20): 2883-2888
URL: https://www.wjgnet.com/1007-9327/full/v13/i20/2883.htm
DOI: https://dx.doi.org/10.3748/wjg.v13.i20.2883