Generation of human/rat xenograft animal model for the study of human donor stem cell behaviors in vivo

Figure 1 A representative two-color flow cytometric analysis for human cell engraftment in PB of human-rat chimeras. After birth, at the indicated time points, peripheral blood (PB) was collected, and subsequently the collected blood cells were stained with both anti-human CD45 and anti-human CD20 antibodies and analyzed with flow cytometry. Human PB from healthy volunteers was employed as positive control (PC). Negative control (NC): normal rat PB.