Generation of human/rat xenograft animal model for the study of human donor stem cell behaviors in vivo

Table 2 Engraftment of human CD45⁺ cells in PB of MNC-transplanted recipients

ExperimentNo.	AnimalNo.	Phenotype	Source	Cell dose	Chimeric/totalanimals
1	661	MNCs	hUCB	5 × 106	8/12
2	662	MNCs	hUCB	5 × 106	5/8
3	670	MNCs	hUCB	5 × 106	6/9

Three weeks after birth, PB was collected from MNC-transplanted rats, followed by measuring the expression of human CD45 maker using flow cytometry to screen human-rat chimeras. Detection of human cells by flow cytometry was defined by the presence of human CD45⁺ cells. No human marker-positive cells were detected in normal rats of the same age. hUCB: Human umbilical cord blood; MNCs: Low-density mononuclear cells. Other details are as in Figure 1.