Cloning and expression of special F protein from human liver

Figure 10 Western-blot of purified human liver F protein. Lane 1: low molecular weight marker; lane 2: precipitation of inclusion granule cleavage; lane 3: inclusion granule dissolved by carbamide; lane 4: supernatant of expression strain after cleavage and centrifugation; lane 5: expression of strain not induced; lane 6: protein of induced expression strain containing the blank vector (pET-15b, not containing exogenous gene F); lane 7: whole proteins of induced blank expression strain not containing the expression vector; lane 8: protein purified by a His-tag column; lane 9: Western-blot result of purified human liver F protein.