Cloning and expression of special F protein from human liver

Figure 9 Electrophoresis of target protein in all expressed and purified strains. Lane 1: low molecular weight marker; lane 2: whole bacteria induced protein; lane 3: inclusion body precipitation after cleavage and centrifugation; lane 4: supernate from all strains cleaved and centrifuged; lane 5: whole bacterial protein not induced; lane 6: expression strain containing induced blank vector (pET15b); lane 7: induced expression of proteins not containing the expression vector; l8: electrophoresis of the target protein taken out from the gels.