Cloning and expression of special F protein from human liver

Figure 7 SDS-PAGE of pET15b-F under experimental conditions. Lane 1: low molecular weigh protein marker; lane 2: all strains of proteins after inducing the expression vector pET15b-F containing the exogenous gene by IPTG; lane 3: inclusion body precipitation by cleavage after inducing the expression strain containing the exogenous gene; lane 4: supernatant by centrifugation after inducing the expression strain containing the exogenous gene; lane 5: all proteins from strains not induced by IPTG containing the exogenous gene; lane 6: expression of proteins induced by IPTG containing no exogenous F protein gene; lane 7: expression of proteins induced by IPTG in a blank strain containing no expression vector.