PI 3-kinase pathway is responsible for antiapoptotic effects of atrial natriuretic peptide in rat liver transplantation

Figure 3 Effect of ANP-preconditioning on distribution and amount of pAkt in liver by confocal microscopy. Animals were infused with NaCl (Co, A), ANP (B), or a combination of ANP and wortmannin (WM/ANP, C) for 20 min. Livers were snap-frozen, cut into 6 µm slices and stained with rhodamine-conjugated phalloidin (F-actin, cytoskeleton, red), HOECHST dye (nuclei, blue), and anti-pAkt antibody, followed by incubation with Alexa Fluor 488 goat anti-mouse antibody (green) as described before. Liver sections were observed under confocal laser microscope (×63 amplification).