Variable expression of cystatin C in cultured trans-differentiating rat hepatic stellate cells

Figure 5 Effect of PDGF-BB and TGF-β1 on CysC expression in HSCs. A: Cultured HSCs were serum starved to 2 mL/L FCS followed by treatment with or without PDGF-BB or TGF-β1 for 18 and 36 h, respectively. Total RNA (5 µg/lane) was isolated and analyzed by Northern blot for CysC expression. As internal loading controls, the 18S and 28S rRNA as well as signals obtained after hybridization with a GAPDH-specific probe were shown; B: for CysC protein expression, cells were treated with or without PDGF-BB or TGF-β1 for 72 h and protein lysates (20 µg/lane) were subjected to Western blot analysis using an antibody specific for CysC. The expression of β-actin was used as internal protein loading control.