Transplantation of fetal liver epithelial progenitor cells ameliorates experimental liver fibrosis in mice

Figure 4 Proliferation and differentiation of FLEP cells in DEN-treated mice and normal mice. A: Immunohistochemical positive control staining for sry protein; normal male mouse liver with nuclear positivity in the majority of hepatocytes (brown nuclei); B: One month after FLEP cell transplantation and DEN treatment, some cells stained for sry. C: Two months after FLEP cell transplantation and DEN treatment, sry⁺ cells were present in liver parenchyma. D and E: Three months after FLEP cell transplantation and DEN treatment, sry⁺ cells formed numerous mature hepatocytes showed canalicular structures (D), or bile duct epithelial cells in bile duct region, as evidenced by diffusely stained sry+ small epithelial cells in bile duct-like structures (E); F: Three months after FLEP cell transplantation without DEN treatment, scattered sry⁺ cells were observed. Original magnifications, x 200 (A-E) and x100 (D).