Loss of disabled-2 expression is an early event in esophageal squamous tumorigenesis

Figure 2 DAB2 exon-1 promoter hypermethylation. Methylation-specific PCR was done to determine the possibility of promoter methylation-mediated gene silencing of DAB2. Eight of 10 ESCCs showed signal (PCR amplicon) for the unmethylated DAB2 alleles (samples 1, 4-10), while 2 cases showed the presence of both unmethylated and methylated PCR amplicon (samples 2 and 3). Burkitt’s lymphoma cell line Raji was used as a positive control for hypermethylation of DAB2 gene (sample 11). In each sample, lane U represents the unmethylated product, while M represents the methylated product, lane NTC refers to the no template control.