Increased DNA binding activity of NF-kB, STAT-3, SMAD3 and AP-1 in acutely damaged liver

Figure 3 Binding activity of NF-κB in acute liver damage. Wistar rats were intoxicated acutely with CCl₄ and then sacrificed at different time points. Nuclear proteins were extracted from the livers and Gel-shift assay was performed for NF-κB transcription factor. A: Binding DNA activity of NF-κB after CCl₄ intoxication; B: supershift assay using 1-h sample, 1 μL of polyclonal antibody against p65 subunit was added and incubated for 1 h before usual EMSA assay; C: densitometric analysis of results of EMSA assay.