Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells

Figure 7 Real-time PCR analysis of the expression of mRNA for CYP2E1 and CYP3A4 in BMSC and ADSC. The expression of two major drug metabolising enzymes, CYP2E1 (A) and CYP3A4 (B), in BMSC (black bars) and ADSC (clear bars) were quantified by RT-PCR at the initial and final times of the differentiation protocol, and compared with that of the human hepatoma cells HepG2. The agarose gel shows the CYP3A4 expression of ADSC (C). Data are shown as a fold increase in the mRNA level compared to HepG2, and were normalized by hPBGD. Data are the mean ± SE of 3 different cultures of BMSC and ADSC. ^bP < 0.01; ^dP < 0.001.