Promoter hypomethylation and reactivation of MAGE-A1 and MAGE-A3 genes in colorectal cancer cell lines and cancer tissues

Figure 2 Methylation analysis of the MAGE-A1 and MAGE-A3 genes in colorectal cancer cell lines. The promoter region of the MAGE-A1 gene was unmethylated in 26 cell lines (SNU-61, SNU-175, SNU-283, SNU-503, SNU-769A, SNU-769B, SNU-1033, SNU-1197A, SNU-C1, SNU-C2A, SNU-C5, Caco2, COLO201, COLO205, COLO320, DLD-1, HCT-15, HCT-116, HT-29, Lovo, LS174T, NCI-H716, SW403, SW1116, and WiDR cell lines). Unmethylated MAGE-A3 DNA amplifications were found in 26 cell lines (SNU-61, SNU-175, SNU-407, SNU-503, SNU-769A, SNU-769B, SNU-1033, SNU-1047, SNU-1197A, SNU-C1, SNU-C2A, SNU-C4, Caco2, COLO201, COLO205, COLO320, DLD-1, HCT-15, HCT-116, HT-29, Lovo, LS174T, NCI-H716, SW403, SW1116, and WiDR cell lines). Lane M denotes product amplified by primers recognizing a methylated sequence and Lane U denotes the product amplified by primers recognizing an unmethylated sequence, respectively.