Human hepatic sinusoidal endothelial cells can be distinguished by expression of phenotypic markers related to their specialised functions in vivo

Figure 2 Isolated, cultured human hepatic sinusoidal endothelial cells exhibit classical morphology under phase contrast microscopy (A), and stain positively with antibody directed against endothelial phenotypic markers. Images represent immunofluorescent staining of cultured cells using specific primary antibodies in an indirect fluorescent protocol with DAPI nuclear counterstain (blue). Positive staining for vWF (B) is visualised using a Texas Red-labelled secondary antibody, whilst expression of endoglin (CD105, C) and ICAM-2 (D) are visualised with a FITC-conjugated secondary antibody (green).