Detection of YMDD mutation using mutant-specific primers in chronic hepatitis B patients before and after lamivudine treatment

doi:10.3748/wjg.v12.i33.5301

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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Viral Hepatitis

World J Gastroenterol. Sep 7, 2006; 12(33): 5301-5305
Published online Sep 7, 2006. doi: 10.3748/wjg.v12.i33.5301

Table 1 DNA sequence of the primers tested for detection of YMDD mutation

¹Primers	²DNA sequence	Nuceotide
YVDD758	5’-CACTGTTTGGCTTTCAGTTATG (A)	737-758 Sense
YVDD758R	5’-CCCAAAACCACATCATCCAC (T)	758-739 Antisense
YVDD669	5’-GGGCCTCAGTCCGTTTCTCA (T)	650-669 Sense
YIDD	5’-CTGTTTGGCTTTCAGTTATATT (G)	739-760 Sense
YIDD2G	5’-GTTTGGCTTTCAGTTATAGT (TG)	741-760 Sense
YIDD2A	5’-GTTTGGCTTTCAGTTATAAT (TG)	741-760 Sense
YIDD2C	5’-GTTTGGCTTTCAGTTATACT (TG)	741-760 Sense
YIDDR	5’-CCCCCAAAACCACATCATCA (C)	760-745 Antisense
YIDDR2T	5’-CCCCCAAAACCACATCATTA (CC)	760-745 Antisense
YIDDR2A	5’-CCCCCAAAACCACATCATAA (CC)	760-745 Antisense
YIDDR2G	5’-CCCCCAAAACCACATCATGA (CC)	760-745 Antisense
HBV376	5’-GATGTGTCTGCGGCGTTTTA	376-395 Sense
HBV485	5’-CCAGGAACATCAACCACCAG	485-504 Sense
HBV1021R	5’-CAGCAAAGCCCAAAAGACCCAC	1021-1000 Antisense

¹ The primers finally selected are indicated in boldface; ² The engineered mismatched nucleotides are underlined; the wild-type nucleotides are shown in parenthesis.

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Citation: Lee CZ, Lee HS, Huang GT, Yang PM, Sheu JC. Detection of YMDD mutation using mutant-specific primers in chronic hepatitis B patients before and after lamivudine treatment. World J Gastroenterol 2006; 12(33): 5301-5305
URL: https://www.wjgnet.com/1007-9327/full/v12/i33/5301.htm
DOI: https://dx.doi.org/10.3748/wjg.v12.i33.5301

Lee CZ, Lee HS, Huang GT, Yang PM, Sheu JC. Detection of YMDD mutation using mutant-specific primers in chronic hepatitis B patients before and after lamivudine treatment. World J Gastroenterol 2006; 12(33): 5301-5305 [PMID: 16981258 DOI: 10.3748/wjg.v12.i33.5301]

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