5-Fluorouracil-related enhancement of adenoviral infection is Coxsackievirus-adenovirus receptor independent and associated with morphological changes in lipid membranes

Figure 2 The number of GFP expression cells was measured by flow cytometry in SW480 and Lovo cells (A). Treatment with 5-FU resulted in increased number of GFP expression cells and increased intensity of GFP expression in both cell lines. Intracellular adenoviral fiber protein was assessed by western blot analysis of SW480 cell lysates (B) after Ad-GFP treatment. In control cells and 5-FU treated cells no fiber protein could be detected. Low amounts of fiber protein could be seen in Ad-GFP treated cells dependent on Ad-GFP concentrations (MOI of 200 and 50). Additional 5-FU treatment increased fiber protein in whole cell lysates indicating enhanced adenoviral uptake. Function of CAR was blocked by anti CAR antibody at a concentration of 1:500 but 5-FU enhanced the number of GFP expressing cells even at anti CAR antibody concentrations of 1:50 (C).