HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro

Figure 5 Testing translation of viral E1 in supernatant and lysates of HepG2 cells infected with HCV from 1 mo culture by Western blot analysis. Supernatant (strip 1) and lysates (strip 2) of HepG2 cells infected with HCV were subjected to Western blot analysis, hybridization with the anti-E1 antibody clearly showed the expression of a cluster of immunogenic proteins at molecular weights localized between 31 and 45 kDa. This cluster was undetectable on the strip immobilized with uninfected HepG2 cell lysates (strip 3).