Cortex cinnamomi extract prevents streptozotocin- and cytokine-induced β-cell damage by inhibiting NF-κB

Figure 2 Inhibition of NF-κB activation in pancreas by CCE pretreatment. Mice were injected with citrate buffer, STZ, or STZ plus CCE. Nuclear proteins isolated from pancreas taken at 30 min after STZ injection (100 mg/kg) were analyzed by EMSA. To confirm reaction specificity, the same experiment was done in the presence of a 50-fold excess of unlabeled oligo probes. Representative result from three independent experiments is shown. lane 1: negative control using 50-fold excess of κB oligonucleotide as a negative competitor; lane 2:citrate buffer; lane 3: STZ; lane 4:STZ + 100 mg/kg CCE; lane 5: STZ + 250 mg/kg CCE; lane 6, STZ + 500 mg/kg CCE.