Anti-inflammatory effects of bifidobacteria by inhibition of LPS-induced NF-κB activation

Figure 3 Microscopic examination of cellular viability. Trypan blue exclusion was monitored in HT-29 clone 34 cells challenged with LPS + HM for 16 after pre-incubation with bifidobacteria (B. longum NCC2705 or B. bifidum S17; moi = 100). Positive control (pos) was cells incubated with LPS + HM without bacterial pre-treatment. As a control for trypan blue staining cells were incubated for 16 h in cell culture medium at pH 4 (dead).