Activation and dramatically increased cytolytic activity of tumor specific T lymphocytes after radio-frequency ablation in patients with hepatocellular carcinoma and colorectal liver metastases

Figure 3 Adenylate kinase (AK) release of cultured tumor cells. Release of adenylate kinase (AK) from the human hepatoma cell line HepG2 and the hman colorectal carcinoma cell line CaCo co-incubated with PBMC isolated before, 4 and 8 wk after RFA. The release of AK correlates with the cytotoxic and NK T cell mediated damage to the cultured cells. HepG2 cells were used for PBMC from the patients with HCC and CaCo cells for the patients with metastasis of colorectal carcinoma.