1,2,3,4,6-penta-O-galloyl-β-D-glucose up-regulates heme oxygenase-1 expression by stimulating Nrf2 nuclear translocation in an extracellular signal-regulated kinase-dependent manner in HepG2 cells

Figure 4 Effects of PGG-induced Nrf2 activation on HO-1 expression and t-Bt-HP-induced toxicity in HepG2 cells. A: Normal cells and the cells transiently transfected with Nrf2 siRNA were pre-incubated with 20 µmol/L PGG for 12 h. HO-1 expression was determined by a flow cytometry. BG indicates background auto-fluorescence; B: Cells and transfected cells pre-incubated for 12 h with 20 µmol/L PGG were exposed to 100 µmol/L t-Bt-HP for 4 h. Data are expressed as mean ± SE of five independent experiments.