Selective inhibition of cell growth by activin in SNU-16 cells

Table 1 Oligonucleotide sequences of PCR primers

Oligo	Sequence	Annealing temperature(°C)	Size (bp)
Inhibin α	5'-AGGAAGAGGAGGATGTCTCC-3'	50	823
	5'-GAGTAACCTCCATCCGAGGT-3'
Activin βA	5'-CTGAACGCGATCAGAAAGCT-3'	54	1014
	5'-TCCTCCACGATCATGTTCTG-3'
Activin βB	5'-TTCGCCGAGACAGATGGCCT-3'	59	765
	5'-AATCATGTTGGGCACGTCCC-3'
ActR IA	5'-GCCCAAGGTCAACCCCAAACTCT-3'	60	265
	5'-GGATTTTCCTTTAGTGGGCAGCT-3'
ActR IB	5'-ACCAGCTGCCTCCAGGCCAACTA-3'	62	245
	5'-GTGCTCAGGCTCCTTGAGGTGAC-3'
ActR IIA	5'-ACCAGTGTTGATGTGGATCTT-3'	60	456
	5'-TACAGGTCCATCTGCAGCAGT-3'
ActR IIB	5'-CATGGAACGAACTGTGTCAT-3'	62	730
	5'-AGATCCACTGAGTCTGGAGA-3'
Smad2	5'-TAGGTGGGGAAGTTTTTGCT-3'	60	410
	5'-TTTGCATGGGACTTGATTGG-3'
Smad4	5'-AAGGTGAAGGTGATGTTTG-3'	60	509
	5'-TAAGGGCCCCAACGGTAAA-3'
Smad7	5'-GTGGGGAGGCTCTACTGTGTC-3'	58	477
	5'-GGCTACCGGCTGTTGAAGATG-3'
p21CIP1/WAF1	5’-CCTCTTCGGCCCAGTGGAC-3’	60	369
	5'-CCGTTTTCGACCCTGAGAG-3'
β-actin	5'-TACCTCATGAAGATCCTCA-3	50	267
	5'-TTCGTGGATGCCACAGGAC-3'

ActR: activin receptor. ActR: activin receptor.