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©2006 Baishideng Publishing Group Co.
World J Gastroenterol. May 21, 2006; 12(19): 3000-3005
Published online May 21, 2006. doi: 10.3748/wjg.v12.i19.3000
Published online May 21, 2006. doi: 10.3748/wjg.v12.i19.3000
Table 1 Oligonucleotide sequences of PCR primers
| Oligo | Sequence | Annealing temperature(°C) | Size (bp) |
| Inhibin α | 5'-AGGAAGAGGAGGATGTCTCC-3' | 50 | 823 |
| 5'-GAGTAACCTCCATCCGAGGT-3' | |||
| Activin βA | 5'-CTGAACGCGATCAGAAAGCT-3' | 54 | 1014 |
| 5'-TCCTCCACGATCATGTTCTG-3' | |||
| Activin βB | 5'-TTCGCCGAGACAGATGGCCT-3' | 59 | 765 |
| 5'-AATCATGTTGGGCACGTCCC-3' | |||
| ActR IA | 5'-GCCCAAGGTCAACCCCAAACTCT-3' | 60 | 265 |
| 5'-GGATTTTCCTTTAGTGGGCAGCT-3' | |||
| ActR IB | 5'-ACCAGCTGCCTCCAGGCCAACTA-3' | 62 | 245 |
| 5'-GTGCTCAGGCTCCTTGAGGTGAC-3' | |||
| ActR IIA | 5'-ACCAGTGTTGATGTGGATCTT-3' | 60 | 456 |
| 5'-TACAGGTCCATCTGCAGCAGT-3' | |||
| ActR IIB | 5'-CATGGAACGAACTGTGTCAT-3' | 62 | 730 |
| 5'-AGATCCACTGAGTCTGGAGA-3' | |||
| Smad2 | 5'-TAGGTGGGGAAGTTTTTGCT-3' | 60 | 410 |
| 5'-TTTGCATGGGACTTGATTGG-3' | |||
| Smad4 | 5'-AAGGTGAAGGTGATGTTTG-3' | 60 | 509 |
| 5'-TAAGGGCCCCAACGGTAAA-3' | |||
| Smad7 | 5'-GTGGGGAGGCTCTACTGTGTC-3' | 58 | 477 |
| 5'-GGCTACCGGCTGTTGAAGATG-3' | |||
| p21CIP1/WAF1 | 5’-CCTCTTCGGCCCAGTGGAC-3’ | 60 | 369 |
| 5'-CCGTTTTCGACCCTGAGAG-3' | |||
| β-actin | 5'-TACCTCATGAAGATCCTCA-3 | 50 | 267 |
| 5'-TTCGTGGATGCCACAGGAC-3' |
- Citation: Kim YI, Lee HJ, Khang I, Cho BN, Lee HK. Selective inhibition of cell growth by activin in SNU-16 cells. World J Gastroenterol 2006; 12(19): 3000-3005
- URL: https://www.wjgnet.com/1007-9327/full/v12/i19/3000.htm
- DOI: https://dx.doi.org/10.3748/wjg.v12.i19.3000