Identification of serum proteins discriminating colorectal cancer patients and healthy controls using surface-enhanced laser desorption ionisation-time of flight mass spectrometry

Table 1 Classification trees generated with the Biomarker Patterns Software

Tree	Included m/z’s (×103 Da): cut-off intensity values and class assignment				Sensitivity (%)	Specificity (%)
	Node 1	Node 2	Node 3	Node 4
I	3.3 ≤ 15.035 Colorectal cancer	-	-	-	77.8	73.3
II	28 ≤ 1.558 Colorectal cancer	4.5 ≤ 29.791 to Node 3	3.1 ≤ 9.866 to Node 4	6.6 ≤ 33.233 Colorectal cancer	77.8	73.3
III	3.3 ≤ 12.757 Colorectal cancer	-	-	-	66.7	83.3
IV	3.3 ≤ 12.757 Colorectal cancer	28 ≤ 1.285 Colorectal cancer	-	-	75.0	83.3
V	3.3 ≤ 12.981 Colorectal cancer	4.5 ≤ 28.599 Healthy control	-	-	84.2	83.3
VI	28 ≤ 1.529 Colorectal cancer	4.5 ≤ 28.577 to Node 3	6.6 ≤ 44.685 Colorectal cancer	-	89.5	88.9

Sensitivities and specificities for trees I to IV were obtained by blinded testing with data from the remaining sample set.