Berberine induces cell cycle arrest and apoptosis in human gastric carcinoma SNU-5 cell line

Figure 7 Changes of levels of p53(A), Bcl-2(B), Bax(C), and cytochrome C (D) in SNU-5 cells after exposure to berberine. SNU-5 cells (5×10⁶/mL) were treated with 0, 25, 50, 100, and 200 μmol/L berberine for 24 h, then cytosolic fraction and total protein were determined as described in Materials and Methods. The levels of p53, p21, Bcl-2, Bax, and cytochrome C were determind by Western blotting as described in Materials and Methods.