Cost-effective method of siRNA preparation and its application to inhibit hepatitis B virus replication in HepG2 cells

Figure 6 Inhibition of HBsAg expression in SMMC7721 cells. SMMC7721 cells were co-transfected with 0.4 μg siHBVP and 0.4 μg pEGFP-HBS (HBS+siHBVP) or 0.4 μg pSEAP2-control (SEAP+siHBVP). As control cells were transfected with 0.4 μg siAP and 0.4 μg pEGFP-HBS (HBS+siAP) or 0.4 μg pSEAP2-control (SEAP+siAP). The positive control group was co-transfected with 0.4 μg pEGFP-HBS and 0.4 μg pSEAP2-control (SEAP+HBS). 48 h post transfection. The supernatants were collected for measuring expression level of secreted alkaline phosphatase, and the cells were lysed with RIPA Buffer for measuring HBsAg expression. A: shows the SEAP and HBsAg expression level represented by OD values; B, C: show the HBsAg or SEAP expression level relative to the positive control. Values represent averages of three independent experiments, with the error bars indicating the standard deviation.