Cost-effective method of siRNA preparation and its application to inhibit hepatitis B virus replication in HepG2 cells

doi:10.3748/wjg.v11.i9.1297

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Mar 7, 2005 (publication date) through May 19, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. Mar 7, 2005; 11(9): 1297-1302
Published online Mar 7, 2005. doi: 10.3748/wjg.v11.i9.1297

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Figure 3 Expression and purification of recombinant E. coli RNase III. A: E. coli RNase III was expressed as an N-terminal His-tagged fusion protein in E. coli strain BL21(DE3). Lane 1: negative control; lanes 2-4 represent 3 individual clones induced with IPTG; B: The recombinant protein extract (Lane 1) was purified by a single step affinity chromatography with the Ni-NTA His•Bind Resin (Lane 2). Molecular weight of marker proteins is indicated nearby.

Citation: Qian ZK, Xuan BQ, Min TS, Xu JF, Li L, Huang WD. Cost-effective method of siRNA preparation and its application to inhibit hepatitis B virus replication in HepG2 cells. World J Gastroenterol 2005; 11(9): 1297-1302
URL: https://www.wjgnet.com/1007-9327/full/v11/i9/1297.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i9.1297