Cost-effective method of siRNA preparation and its application to inhibit hepatitis B virus replication in HepG2 cells

Figure 1 Sequences of primer 2p5 and 2p3. The last 18 nt of 2p5 correspond to the antisense strand before Xho I site of pET-22b, the nucleotide sequence in gray box of 2p5 correspond to the tac promoter, and the underlined letters represent the Xho I site. The last 21 nt of 2p3 correspond to the sense strand after the Bgl I site of pET-22b, the nucleotide sequence in gray box of 2p3 correspond to the T3 terminator, and the underlined letters represent the Bgl II site.