Polymorphisms of uridine-diphosphoglucuronosyltransferase 1A7 gene in Taiwan Chinese

Table 5 Natural or mutagenesis primers, restriction enzymes and the results for UGT1A7 variations.

Position (cDNA)	Primers	Sequence	Restriction enzyme	Result (bp)
-57 T→G	U7F1	5’ TGAATGAATAAGTACACGCC	HpyCH4IV	N2	439
	U7R1	5’ ATAGAGAAAATGCACTTCGC3’		V3	57+382
33 C→A	1stPCR
	U7F1	5’ TGAATGAATAAGTACACGCC
	U7R2	5’ TAGGGGCAAAAAATGTTC3’
	2ndPCR
	U7-33F1	5’ GGGTGGACTGGCCTCCTG3’1	Taq I	N	115
	U7-33R	5’ ACTGCATGGTGAACCAGTCG		V	19+96
387 T→G	1stPCR
	U7F1	5’ TGAATGAATAAGTACACGCC
	U7R2	5’ TAGGGGCAAAATAAATGTTC
	2ndPCR
	U7-387F	5’ AAATTGCAGGAGTTCTTA3’1	Afl II	N	159
	U7-387R	5’ TGGCAAAATATTCCCCGC3’		V	140+19
622 T→C	U7F3	5’ TGTCCCCAGACTTCTCTTAG	Rsa I	N	447
	U7R3	5’ GCTACCCAACAATTAAGTGA		V	54+393

bp: base pair,

¹mutagenesis site,

²N: digestion result of wild type,

³V: digestion result of variant.