High affinity mouse-human chimeric Fab against Hepatitis B surface antigen

doi:10.3748/wjg.v11.i48.7569

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Dec 28, 2005 (publication date) through May 22, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. Dec 28, 2005; 11(48): 7569-7578
Published online Dec 28, 2005. doi: 10.3748/wjg.v11.i48.7569

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Figure 3 SDS-PAGE (A) and Western Blot (B) analysis of the chimeric Fab. The purified chimeric Fab was resolved in 12% SDS-PAGE under reducing (R) and non-reducing conditions (NR). In non-reducing conditions the Fab is a heterodimer of molecular weight ~50 ku. In reducing conditions the heterodimer dissociates into chimeric light chain (~25 ku) and Fd (~25 ku). Both the gels were stained by silver staining. M is the lane for the protein marker. Similar observations were made in Western Blots (B) of the chimeric Fab in reducing (R) and non-reducing (NR) conditions. Electroblotted antibody fragments were detected by Rabbit anti-Human IgG-HRP conjugate (1:1 000 dilution; Dako).

Citation: Bose B, Khanna N, Acharya SK, Sinha S. High affinity mouse-human chimeric Fab against Hepatitis B surface antigen. World J Gastroenterol 2005; 11(48): 7569-7578
URL: https://www.wjgnet.com/1007-9327/full/v11/i48/7569.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i48.7569