Detection and identification of intestinal pathogenic bacteria by hybridization to oligonucleotide microarrays

Table 2 Oligonucleotide probes used in the present study

No.	Sequence (5' to 3')	Target
1	gtacaaggcccgggaacgtattcacc	All known eubacteria (universal bacterial probe)
2	gacataaggggcatgatgatttgacgt	All Gram-positive bacteria
3	gtcgtaagggccatgatgacttgacgt	All Gram-negative bacteria
4	gtcatgaatcacaaagtggtaagcgc	All enteric bacterial
5	acgacgcactttatgaggtccgcttg	Escherichia coli, Shigella sp. and Salmonella sp.
6	gctcctaaaaggttactccaccggct	Staphylococcus aureus
7	cgacggctagctccaaatggttactg	Coagulase-negative Staphylococcus
9	tcacggtcttgcgtcttattgtacctac	Clostridium botulinum
11	gaactgagactggtttttaagtttggct	Clostridium perfringens
15	cgaactgggacatattttatagatttgc	Campylobacter jejuni
16	aggtcgccccttcgccgccctctgtatc	Legionella pneumophila
17	cgatccgaactgagaccggcttttaagg	Mycobacterium tuberculosis
18	tactcgtaagggccatgatacgacttaa	Proteus sp.
19	cgcggcttggcaaccctttgtaccgacc	Pseudomonas aeruginosa
20	actgagaatagttttatgggattagg	Listeria monocytogenes
21	gctccaccttcgcggtattcgctgccct	Vibrio cholerae
22	tcactttcgcaagttggccgccctctgt	Vibrio fluvialis
23	tggtaagcgtccccccgtagttgaaac	Vibrio parahaemolyticus
24	tacgacagactttatgtggtccgcttgc	Yersinia enterocolitica
25	cctcgcggtctagcagctcgttgtgctt	Enterococcus faecalis
26	ggattcgctcactatcgctagcttgcag	Aeromonas hydrophila
27	ccgacttcgggtgttacaaactctcg	Bacillus cereus, P.
28	gcttcatgcactcgagttgcagagtg	cocovenenans subsp. farinofermentans
30	atccccaccttcctccagtt	Positive control
31	cccccagaggcagagattgca	virA gene of Shigella sp.
32	cgccaataacgaattgcccga	invA gene of Salmonella sp.