Lewis blood genotypes of peptic ulcer and gastric cancer patients in Taiwan

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World Journal of Gastroenterology

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1007-9327

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World J Gastroenterol. Aug 21, 2005; 11(31): 4891-4894
Published online Aug 21, 2005. doi: 10.3748/wjg.v11.i31.4891

Table 1 Primer sequences and restriction enzymes of mutation in Se and Le genes

Mutation	Primer sequence	Enzyme
Se gene
A385T	UP¹: GATGGAGGAGGAATACCGCCTC	Ear I
	DP²: GATCTCCTGGCGGAGGTGGTGGTAGAAGATC
G428A	Identical to A358T primer pairs	Bgl II
C571T	UP: AGGAGATCCTCCAGGAGTTCA	Dde I
	DP: AGAAGGAGAAAAGGTCTCAAAGG
G849A	Identical to C571T primer pairs	Dde I
C628T	UP: AGTGTGGAAGGGGGTGGTGCCC	Bgl I
	DP: CCACTCTGGCAGGAAGGC
Fusion gene	UP: CTGCCTCCTGACCATGTCC	Pst I
	DP: identical to reverse primer of C628T
Le genes
T202C and C314T	UP: CCACCCTCCTGATCCTGCTC	Msp I (T202C)
	DP: GATATCCCAGTGGTGCACGATGATGATC	Bcl I (C314T)
C445A	UP: identical to UP of T202 C and C314 T	BstN I
	DP: GAGATTGAAGTATCTGTCCAAGGC
G508A	UP: TCAACTTGGAGCCACACCCT	Alu I
	DP: AGTTGGACACCGCCCAGGCCACCAG
A1007C	UP: GCTCCTTCCGCTGGGCACTAG	Alu I
	DP: TGGCCACAAAGGACTCCAGC
T1067A	UP: GTACCAGACGGTGCGATGCA	Nsi I
	DP: identical to DP of A1007 C

¹UP: upstream primer; ²DP: downstream primer; The mutated base is underlined.

Citation: Yei CJ, Chang JG, Shih MC, Lin SF, Chang CS, Ko FT, Lin KY, Liu TC. Lewis blood genotypes of peptic ulcer and gastric cancer patients in Taiwan. World J Gastroenterol 2005; 11(31): 4891-4894
URL: https://www.wjgnet.com/1007-9327/full/v11/i31/4891.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i31.4891

Yei CJ, Chang JG, Shih MC, Lin SF, Chang CS, Ko FT, Lin KY, Liu TC. Lewis blood genotypes of peptic ulcer and gastric cancer patients in Taiwan. World J Gastroenterol 2005; 11(31): 4891-4894 [PMID: 16097066 DOI: 10.3748/wjg.v11.i31.4891]

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