Biological impacts of &ldquo;hot-spot&rdquo; mutations of hepatitis B virus X proteins are genotype B and C differentiated

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 11, Issue 30

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. Aug 14, 2005; 11(30): 4703-4708
Published online Aug 14, 2005. doi: 10.3748/wjg.v11.i30.4703

Table 1 Sequences and related applications of the primers used for site-directed mutagenesis and nomenclature of HBx mutants

Primers	Sequences (5’→3’)	Applications		Nomenclature of the HBx mutants
Primers	Sequences (5’→3’)	Nucleotide mutation²	Amino acid substitution³	Nomenclature of the HBx mutants
R	CTCCTCCCCCAACTCTTCCCACTCAGTAAAC
F1	GAAGAGTTGGGGGAGGAGAC¹	1753^T→C	I127T	XB/XC-127
	TAGGTTAAAGGTC
F2	GAAGAGTTGGGGGAGGAGACTAGGTTA	1753^T→C + 1762^A→T + 1 764^G→A	I127T + K130M + V131I	XB/XC-127-130-131
	ATGATCTTTGTACTAGG
F3	GAAGAGTTGGGGGAGGAGATTAGGTTA	1762^A→T + 1764^G→A	K130M + V131I	XB/XC-130-131
	ATGATCTTTGTACTAGG
F4	GAAGAGTTGGGGGAGGAGATTAGGTTA	1762^A→T + 1764^G→A + 1768^T→A	K130M + V131I + F132Y	XB/XC-130-131-132
	ATGATCTATGTACTAGGAGGC
F5	GAAGAGTTGGGGGAGGAGATTAGGTTA	1768^T→A	F132Y	XB/XC-132
	AAGGTCTATGTACTAGGAGGC

¹Mutated nucleotides are shown as underlined;

²1 753, 1 762, 1 764, 1 768 indicate the positions of mutated nucleotides in HBV genome;

³127, 130, 131, 132 are positions of substituted amino acids in HBx.

Citation: Lin X, Xu X, Huang QL, Liu YQ, Zheng DL, Chen WN, Lin JY. Biological impacts of “hot-spot” mutations of hepatitis B virus X proteins are genotype B and C differentiated. World J Gastroenterol 2005; 11(30): 4703-4708
URL: https://www.wjgnet.com/1007-9327/full/v11/i30/4703.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i30.4703

Lin X, Xu X, Huang QL, Liu YQ, Zheng DL, Chen WN, Lin JY. Biological impacts of “hot-spot” mutations of hepatitis B virus X proteins are genotype B and C differentiated. World J Gastroenterol 2005; 11(30): 4703-4708 [PMID: 16094714 DOI: 10.3748/wjg.v11.i30.4703]

All content on this site: Copyright © 1993-2026 Baishideng Publishing Group Inc, its licensors, and contributors. All rights are reserved, including those for text and data mining, AI training, and similar technologies. For all open access content, the relevant licensing terms apply.