Colorectal Cancer
Copyright ©The Author(s) 2005.
World J Gastroenterol. Jul 7, 2005; 11(25): 3871-3876
Published online Jul 7, 2005. doi: 10.3748/wjg.v11.i25.3871
Table 2 Time events for the switching of column and of mobile phase1
Time (min)PumpEventValve position
0.00 – 7.00Pump 1 (eluent A)Sample matrix are discharged by column 11
Pump 2 (eluent B)Conditioning of column 2
7.00 – 10.00Pump 2 (eluent B)Analytes are transferred from column 1 to column 22
10.00 – 50.00Pump 2 and Pump 3 (eluents B and C)Analysis of nucleosides on column 2 by using a linear1
gradient elution program
Pump 1 (eluent A)Conditioning of column1