Different cytokine response of primary colonic epithelial cells to commensal bacteria

Figure 6 L. rhamnosus (LGG) interferes with E. coli- and B. ovatus-induced cytokine production by CEC. CEC were cultured for 4 h with individual bacteria alone or with a mixture of equal numbers of two different bacteria (B. ovatus+ E. coli or E. coli+L. rhamnosus) such that the total number of bacteria in each culture was the same. A: KC mRNA and protein expression by RT-PCE and ELISA; B: IL-6 mRNA and protein expression by RT-PCR and ELISA. ELISA data was obtained by combining the data sets from three independent experiments. Error bars designate 95% confidence limits.