Different cytokine response of primary colonic epithelial cells to commensal bacteria

doi:10.3748/wjg.v11.i22.3375

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Jun 14, 2005 (publication date) through Apr 25, 2024

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Times Cited (44)

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Research

World J Gastroenterol. Jun 14, 2005; 11(22): 3375-3384
Published online Jun 14, 2005. doi: 10.3748/wjg.v11.i22.3375

Table 1 PCR primer sequences.

Gene	Forward 5'-3’	Reverse 5'-3’
Cytokines
IL-1b	GAGATTGAGCTGTCTGCTCA	AAGGAGAACCAAGCAACGAC
IL-6	GATGCTACCAAACTGGATATAATC	GGTCCTTAGCCACTCCTTCTGTG
IL-18	ACTGTACAACCGCAGTAATACGG	AGTGAACATTACAGATTTATCCC
TNFa	TGGGAGTAGACAAGGTACAACCC	CATCTTCTCAAAATTCGAGTGACAA
ENA78 (CXCL5)	CTTCCTCAGTCATAGCCGCAAC	ATCCGTGGGTGGAGAGAATCAG
IP-10 (CXCL10)	TTTCTGCCTCATCCTGCTGG	GGAGCCCTTTTAGACCTTTTTTGG
MIP-1a (CCL3)	GCCCTTGCTGTTCTTCTCTGT	GGCATTCAGTTCCAGGTCAGT
MIP-1b (CCL4)	ACACCATGAAGCTCTGCGT	CGCTGGAGCTGCTCAGTTC
MIP-2a (CXCL2)	CAAAGGCAAGGCTAACTG	TGTTCTACTCTCCTCGGT
MIP-3a (CCL-20)	GCAGAAGCAGCAAGCAACTACG	GAGGTTCACAGCCCTTTTCACC
MCP-1 (CCL2)	CTCACCTGCTGCTACTCATTC	GCTTGAGGTGGTTGTGGAAAA
MCP-3 (CCL7)	TGTGCCTGAACAGAAACCAACC	AAAAATGGGGAAAGGGGGAG
KC (GRO)	GTCCTTTGAACGTCTCTGTC	GCTGGCTTCTGACAACACTA
RANTES (CCL5)	CAAGGAGTATTTCTACACCAGCAGC	ATGCCGATTTTCCCAGGACC
MEC (CCL-28)	GCTGTGTGTGTGGCTTTTCAAAC	TTCTGTCCTTCCTGCTGGGTTG
TLR- signaling
MyD88	ACCCCACTCGCAGTTTGTTGGATG	TGGTGATGCCTCCCAGTTCCTTTG
TIRAP	CAATCTACCTGGAATCGGCTGTC	GCATCTTCTTGGGCTTCTTCAAC
IRAK	TGCCGCTTCTACAAAGTGATGGACG	AATGGGTCTGGGAGCCTGGAAAAG
Caspase-1	ATTACTGCTATGGACAAGGCACGG	CCCTCGGAGAAAGATGTTGAAACTC
TRAF-6	AATGGAAGCACAGCAGTGTAACGGG	AAGGCAAGCAGTTCTGGTTTGGCG
IKKb	TGGAGCCTGGGAAATGAAAGAACG	TCTAAGAGCCGATGCGATGTCACTC
IKKa	AGCCATTCCTCACTTACCAGTCCG	CAGCAGTCTCTTCCACAGAAAACCC
Others
CD45	CTTCAGAGCCTCGTACCAGC	TGTGTCCAGAAGGGCAAATC
b-actin	GCTTCTTTGCAGCTCCTTCGTTG	TTCTCCATGTCGTCCCAGTTGG
Vimentin	CTGTGTCCTCGTCCTCCTACC	GCAGTTCTACCTTCTCGTTGG
b-defensin3	GCATTTGAGGAAAGGAACTCCACAAC	GTCTCCACCTGCAGCTTTTAGCAA
Angiogenin4	CTCTGGCTCAGAATGTAAGGTACGA	GAAATCTTTAAAGGCTCGGTACCC
SAA-1	GTAATTGGGGTCTTTGCC	TTCTGCTCCCTGCTCCTG

Citation: Lan JG, Cruickshank SM, Singh JCI, Farrar M, Lodge JPA, Felsburg PJ, Carding SR. Different cytokine response of primary colonic epithelial cells to commensal bacteria. World J Gastroenterol 2005; 11(22): 3375-3384
URL: https://www.wjgnet.com/1007-9327/full/v11/i22/3375.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i22.3375