Viral Hepatitis
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. May 28, 2005; 11(20): 3060-3064
Published online May 28, 2005. doi: 10.3748/wjg.v11.i20.3060
Figure 3
Figure 3 Purification of thio-PreS. A: Fractions of thio-PreS eluted with increasing imidazole concentrations listed at the bottom of each lane. The triangle indicates the position of thio-PreS; B: Further purification with a freeze-thaw treatment. Lanes 1 and 2: supernatants of the bacteria lysate before or after IPTG induction, respectively; lanes 3 and 4: supernatant and precipitate of the affinity purified thio-PreS sample after a freeze-thaw treatment. Molecular weight maker is denoted as M.