TIP30 regulates apoptosis-related genes in its apoptotic signal transduction pathway

Figure 3 FACS analyses of annexin-V-FITC and PI double-stained cells. Cells were infected with Ad-TIP30 and mock as control (no virus). After treatment for 12, 24, 36 h, cells were double-stained at various indicated time points with annexin-V-FITC and PI and analyzed by flow cytometry. Cells appearing in the lower right quadrant showed positive annexin-V-FITC staining, which indicated phosphatidylserine translocation to the cell surface and no DNA staining by PI. Data in the figures are mean±SD. The percentage of apoptosis cells of three cell lines treated as previously (P<0.01).