Polymerase chain reaction-single strand conformational polymorphism analysis of rearranged during transfection proto-oncogene in Chinese familial hirschsprung’s disease

Table 1 Primers used in PCR amplification and DNA sequencing experiments.

Exon	Sequence (5'→3')	Annealing t (°C)	Product size (bp)
11	F: ACACCACCCCCACCCACAGAT	62	273
	R: AAGCTTGAAGGCATCCACGG
13	F: GACCTGGTATGGTCATGGA	58	253
	R: AAGAGGGAGAACAGGGCTGTA
15	F: GACTCGTGCTATTTTTCCTAC	60	234
	R: TATCTTTCCTAGGCTTCCC
17	F: CCCCACTAGATGTATAAGGG	59	232
	R: TCACTGGTCCTTTCACTCTCT

F: forward primer; R: reverse primer.