Multigene tracking of quasispecies in viral persistence and clearance of hepatitis C virus

Table 2 Primers for amplification of E1 and E2 regions of HCV genome¹.

Region	Designation	Sequence	5’ to 3’ polarity	Position of 5’ base2
C/E2	P1	GCAACAGGGAAYYTDCCCGGTTGCTC	Outer sense	837–862
	P2	TTCATCCASGTRCAVCCRAACCA	Outer antisense	2020–1998
	P3	CCATGTGCTCYTTYTCTATCTTC	Inner sense	855–874
	P4	GTTTAAARCARTACACYGGRCCRCANAC	Inner antisense	1882–1857
C/E1	P3	CCATGTGCTCYTTYTCTATCTTC	Sense	855–874
	P5	GTRGGBGACCAGTTCATCATCAT	Antisense	1333–1311
E2	P6	GGGAYATGATGATGAACTGGTC	Sense	1306–1327
	P4	GTTTAAARCARTACACYGGRCCRCANAC	Antisense	1882–1857

¹Degenerate bases are indicated with standard codes of the international union of Pure and Applied Chemistry.

²Position of 5’ base relative to the HCV genomic sequence of the HCV J6 strain.