Transforming growth factor-β1 gene polymorphisms are associated with progression of liver fibrosis in Caucasians with chronic hepatitis C infection

Table 1 LC Protocol 1 for Genotyping the TGF-β1 Gene Polymorphisms.

Codon 10 (Leu10Pro)
Time (s)	Temp (°C)	Transition Rate (°C/s)	Cycles
600	95	20	1	Denaturation
10	95	20	45	Cycling
10	59	20
20	72	20
60	95	20	1	Melting curve
				Analysis
30	45	20
0	78	0.2
5-d(CTAGGTTATTTCCGTGGG)-3’				Sense primer
5-d(CCTTGGCGTAGTAGTCG)-3’				Reverse primer
5-d(TCCCCCATGCCGCCCTCCGGGCT-Fluo)-3’				Anchor primer
5-LCRed640-(CGGCTGCTGCCGCTGCT-P)-3’				Sensor primer