Hyperammonemia, brain edema and blood-brain barrier alterations in prehepatic portal hypertensive rats and paracetamol intoxication

Figure 4 Trypan blue and BBB. A: Trypan blue dye in hippocam-pus of a sham rat. No breakdown of BBB was observed. Trypan blue was only positive in vascular space (100 ×); B: Trypan blue dye diffusion due to the breakdown of BBB as an example of what was observed in hippocampus in groups II, III and IV (100 ×).