Liver Cancer
Copyright ©The Author(s) 2004.
World J Gastroenterol. May 1, 2004; 10(9): 1281-1285
Published online May 1, 2004. doi: 10.3748/wjg.v10.i9.1281
Table 2 Oligonucleotides used for PCR amplifications
GeneQuantification methodSequencecDNA
MDR1Forward primer5’- CCCAGGAGCCCATCCTGT-3’3774-3791
Revease primer5’- CCCGGCTGTTGTCTCCATA-3’3838-3821
Probe5’-(FAM)TGACTGCAGCATTGCTGAGAACATTGC(TAMRA)-3’3793-3819
MRPForward primer5’- AAGCGCCTCGAGTCGGT-3’3617-3633
Revease primer5’- TCGAATGACGCTGACCCC-3’3694-3677
Probe5’-(FAM)AGCCGCTCCCCGGTCTATTCCC-(TAMRA)-3’3635-3656
LRPForward primer5’- TTTCGATGACTTCCATAAGAACTCA-3’1881-1905
Reverse primer5’- TTCCGAGGTCTCAAAGCCAA-3’1950-1931
Probe5’(FAM)-CCCGCATCATTCGCACTGCTGT- (TAMRA)3’1907-1928
GAPDHForward primer5’- GAAGGTGAAGGTCGGAGTCA-3’
Revease primer5’- GAAGATGGTGATGGGA-3’
Probe5’-(JOE)CAAGCTTCCCGTTCTCAGCC(TAMRA)-3’