Adeno-associated virus mediated endostatin gene therapy in combination with topoisomerase inhibitor effectively controls liver tumor in mouse model

Figure 5 Detection of endostatin in livers of tumor-bearing mice. Hepatic tumors were induced by injecting murine sar-coma cells into the liver. After 7 d, the livers were harvested from the mice of different groups and analyzed by immuno-histochemical staining for endostatin. Livers without treatment, rAAV-LacZ alone and rAAV-LacZ plus etoposide pretreatment did not express endostatin. The vessels of livers treated with rAAV-endostatin were stained with anti-endostatin antibodies and a significant increase was observed in the rAAV-endostatin plus etoposide treatment group (arrow). Endostatin was also detected in hepatocytes of the rAAV-endostatin plus etoposide treatment group (arrow head).