Effect of mitogen-activated protein kinase signal transduction pathway on multidrug resistance induced by vincristine in gastric cancer cell line MGC803

Figure 3 The expression of associated gene of MDR in MGC803 cells treated with VCR or VCR+PD098059 24h-72 h. A: The expression of MDR1 of MGC803 cells treated with VCR increased. B,C: The expression of MRP1 and GST-π of MGC803 cells treated with VCR did not increase. D: The expression of MDR1 was inhibited when MGC803 cells treated with VCR and PD098059. E: The protein level of β-actin was detected to assess the loading amount in each well in SDS-PAGE. Lane 1, untreated cells; Lane 2, positive control cells; Lane 3, cells treated for 24 h; Lane 4, cells treated 48 h; Lane 5, cells treated for 72 h.