Activation of human colon mast cells through proteinase activated receptor-2

Table 3 Inhibition of tc-LIGRLO, SLIGKV, trypsin, anti-IgE or calcium ionophore A23187 (CI) induced histamine release from colon mast cells by pertussis toxin (1 µg/mL) and metabolic inhibitors

Concentration of stimulus		% inhibition of histamine release
		Pertussis toxin	Metabolic inhibitors
tc-LIGRLO	100 µmol/mL	92 ± 5.4a	98 ± 2.0a
SLIGKV	100 µmol/mL	95 ± 5.9a	92 ± 3.8a
Trypsin	10 µg/mL	84 ± 6.8a	88 ± 8.7a
Anti-IgE	10 µg/mL	84 ± 5.2a	99 ± 1.1a
CI	1.0 µg/mL	97 ± 2.4a	99 ± 0.1a

The values shown are mean ± SEM for four separate experiments performed in duplicate.

^aP < 0.05 compared with the uninhibited control group (paired Student’s t test).