Activation of human colon mast cells through proteinase activated receptor-2

Table 2 Inhibition of tc-LIGRLO, SLIGKV, trypsin, anti-IgE or calcium ionophore A23187 (CI) induced tryptase release from colon mast cells by pertussis toxin (1 µg/mL) and metabolic inhibitors

Concentration of stimulus		% inhibition of tryptase release
		Pertussis toxin	Metabolic inhibitors
tc-LIGRLO	100 µmol/mL	85 ± 9.6a	91 ± 9.3a
SLIGKV	100 µmol/mL	91 ± 4.8a	85 ± 9.6a
Trypsin	10 µg/mL	100 ± 0a	91 ± 3.9a
Anti-IgE	10 µg/mL	87 ± 10a	94 ± 3.6a
CI	1.0 µg/mL	84 ± 8.8a	95 ± 4.0a

The values shown are mean ± SEM for four separate experiments performed in duplicate.

^aP < 0.05 compared with the uninhibited control group (paired Student’s t test).