Opisthorchiasis-associated biliary stones: Light and scanning electron microscopic study

Figure 1 Histochemical staining of biliary sludge for bilirubin (A) and calcium (B). Calcium appears as dark deposits on the Opisthorchis eggshell (arrow) and bilirubin precipitates are demonstrated in the outer layer (arrowhead). Normal parasite eggs without deposition are shown in the same field. (A = Fouchet stain, B = von Kossa stain).