Expression of hepatitis C virus envelope protein 2 induces apoptosis in cultured mammalian cells

Figure 6 Apoptosis of CHO/sS1E2 cells. A: Hoechst staining of CHO and CHO/sS1E2 cells 24 h after seeding. Photograph is a representative experiment repeated three times (original magnification 400 × ). The percentage of condensed and fragmented nuclei is indicated at the bottom of each photo. Numbers are presented as mean ± SD. B: Flow cytometry analysis of PI stained cells 24 h after seeding. The percentage of cells with hypodiploid genomic DNA is indicated on each of the histogram. Results were a representative experiment repeated twice. C: Fragmentation of CHO/sS1E2 cell DNA. Lane 1: CHO cells 48 h post-seeding; lane 2: CHO cells freshly seeded; lane 3: CHO/sS1E2 cells 48 h post-seeding; lane 4: CHO/sS1E2 cells freshly seeded; lane 5: DNA size marker.