Expression of hepatitis C virus envelope protein 2 induces apoptosis in cultured mammalian cells

Figure 4 Expression of E2-661 in CHO/sS1E2 cells. A: Immunofluorescence of E2 in CHO/sS1E2 cells analyzed with a mono-clonal anti-preS1 antibody 125E11 (original magnification 400 × ). B: Western blot analysis of the E2 products from CHO/sS1E2 cells. Cell lysates and proteins precipitated from culture medium were analyzed with anti-E2 polyclonal antibody RE2116 after N-glycosidase treatment. Lanes 1, 2, 5, 6: cell lysates from CHO cells; lanes 3, 4, 7, 8: cell lysates from CHO/sS1E2 cells; lanes 9, 10, 13, 14: culture medium from CHO cells; lanes 11, 12, 15, 16: culture medium from CHO/sS1E2 cells; lanes 1, 3, 9, 11: samples incubated with Endo H digestion buffer; lanes 2, 4, 10, 12: samples digested with Endo H; lanes 5, 7, 13, 15: samples incubated with PNGase F digestion buffer; lanes 6, 8, 14, 16, samples digested with PNGase F.